rabbit anti glun2a Search Results


rabbit anti-glun2a/2b  (Synaptic Systems)
90
Synaptic Systems rabbit anti-glun2a/2b
( A ) Top: patch RT-PCR strategy. Bottom: representative voltage responses to current steps in CA1 pyramidal neuron (far left, black), and current responses to voltage steps in astrocytes in stratum radiatum (SR, green), stratum oriens (SO, yellow), and stratum lacunosum moleculare (SLM, blue). Scale bars: neuron, 40 mV, 500 ms; astrocytes, 6 nA, 200 ms. ( B ) Summary of I-V curves of astrocytes obtained before collecting RNA. N = 39 cells, 36 slices, 9 mice for each layer. ( C ) Summary of RT-PCR analysis for mRNA encoding indicated NMDAR subunits: GRIN1 (1), GRIN2A (2A), GRIN2B (2B), and GRIN2C (2C). Control samples (no patch) were obtained by inserting the electrode into the slice without patching cells. GRIN2D levels were undetectable in all cells examined (data not shown). ( D ) Western blots of co-immunoprecipitations performed using adult mouse hippocampal extracts with the indicated antibodies where anti-Cre antibody is used as a negative control. The blots are probed for <t>GluN2A/2B</t> (top row), GluN2C (middle row) or GluN1 (bottom row). ( E ) Representative brain sections of GRIN2C -Cre mice crossed to a tdTomato reporter line (Ai9), showing hippocampal area CA1 immunolabelled for GFAP, tdTomato and NeuN; scale bars, 160 μm (top), 25 μm (bottom). ( F ) Quantification of % cells that are positive for tdTomato amongst NeuN-labelled cells in stratum pyramidale (left) and SR (middle) and GFAP-labelled cells (right).
Rabbit Anti Glun2a/2b, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+glun2a/bio_rxiv__2021__05__28__446253-287-8-10?v=Synaptic+Systems
Average 90 stars, based on 1 article reviews
rabbit anti-glun2a/2b - by Bioz Stars, 2026-07
90/100 stars
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rabbit anti-glun2a  (GeneTex)
90
GeneTex rabbit anti-glun2a
( A ) Top: patch RT-PCR strategy. Bottom: representative voltage responses to current steps in CA1 pyramidal neuron (far left, black), and current responses to voltage steps in astrocytes in stratum radiatum (SR, green), stratum oriens (SO, yellow), and stratum lacunosum moleculare (SLM, blue). Scale bars: neuron, 40 mV, 500 ms; astrocytes, 6 nA, 200 ms. ( B ) Summary of I-V curves of astrocytes obtained before collecting RNA. N = 39 cells, 36 slices, 9 mice for each layer. ( C ) Summary of RT-PCR analysis for mRNA encoding indicated NMDAR subunits: GRIN1 (1), GRIN2A (2A), GRIN2B (2B), and GRIN2C (2C). Control samples (no patch) were obtained by inserting the electrode into the slice without patching cells. GRIN2D levels were undetectable in all cells examined (data not shown). ( D ) Western blots of co-immunoprecipitations performed using adult mouse hippocampal extracts with the indicated antibodies where anti-Cre antibody is used as a negative control. The blots are probed for <t>GluN2A/2B</t> (top row), GluN2C (middle row) or GluN1 (bottom row). ( E ) Representative brain sections of GRIN2C -Cre mice crossed to a tdTomato reporter line (Ai9), showing hippocampal area CA1 immunolabelled for GFAP, tdTomato and NeuN; scale bars, 160 μm (top), 25 μm (bottom). ( F ) Quantification of % cells that are positive for tdTomato amongst NeuN-labelled cells in stratum pyramidale (left) and SR (middle) and GFAP-labelled cells (right).
Rabbit Anti Glun2a, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+glun2a/pmc07059821-54-51-53?v=GeneTex
Average 90 stars, based on 1 article reviews
rabbit anti-glun2a - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
rabbit anti-glun2a-y1325  (Symansis Inc)
90
Symansis Inc rabbit anti-glun2a-y1325
( A ) Top: patch RT-PCR strategy. Bottom: representative voltage responses to current steps in CA1 pyramidal neuron (far left, black), and current responses to voltage steps in astrocytes in stratum radiatum (SR, green), stratum oriens (SO, yellow), and stratum lacunosum moleculare (SLM, blue). Scale bars: neuron, 40 mV, 500 ms; astrocytes, 6 nA, 200 ms. ( B ) Summary of I-V curves of astrocytes obtained before collecting RNA. N = 39 cells, 36 slices, 9 mice for each layer. ( C ) Summary of RT-PCR analysis for mRNA encoding indicated NMDAR subunits: GRIN1 (1), GRIN2A (2A), GRIN2B (2B), and GRIN2C (2C). Control samples (no patch) were obtained by inserting the electrode into the slice without patching cells. GRIN2D levels were undetectable in all cells examined (data not shown). ( D ) Western blots of co-immunoprecipitations performed using adult mouse hippocampal extracts with the indicated antibodies where anti-Cre antibody is used as a negative control. The blots are probed for <t>GluN2A/2B</t> (top row), GluN2C (middle row) or GluN1 (bottom row). ( E ) Representative brain sections of GRIN2C -Cre mice crossed to a tdTomato reporter line (Ai9), showing hippocampal area CA1 immunolabelled for GFAP, tdTomato and NeuN; scale bars, 160 μm (top), 25 μm (bottom). ( F ) Quantification of % cells that are positive for tdTomato amongst NeuN-labelled cells in stratum pyramidale (left) and SR (middle) and GFAP-labelled cells (right).
Rabbit Anti Glun2a Y1325, supplied by Symansis Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+glun2a/pm27796759-88-23-26?v=Symansis+Inc
Average 90 stars, based on 1 article reviews
rabbit anti-glun2a-y1325 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


( A ) Top: patch RT-PCR strategy. Bottom: representative voltage responses to current steps in CA1 pyramidal neuron (far left, black), and current responses to voltage steps in astrocytes in stratum radiatum (SR, green), stratum oriens (SO, yellow), and stratum lacunosum moleculare (SLM, blue). Scale bars: neuron, 40 mV, 500 ms; astrocytes, 6 nA, 200 ms. ( B ) Summary of I-V curves of astrocytes obtained before collecting RNA. N = 39 cells, 36 slices, 9 mice for each layer. ( C ) Summary of RT-PCR analysis for mRNA encoding indicated NMDAR subunits: GRIN1 (1), GRIN2A (2A), GRIN2B (2B), and GRIN2C (2C). Control samples (no patch) were obtained by inserting the electrode into the slice without patching cells. GRIN2D levels were undetectable in all cells examined (data not shown). ( D ) Western blots of co-immunoprecipitations performed using adult mouse hippocampal extracts with the indicated antibodies where anti-Cre antibody is used as a negative control. The blots are probed for GluN2A/2B (top row), GluN2C (middle row) or GluN1 (bottom row). ( E ) Representative brain sections of GRIN2C -Cre mice crossed to a tdTomato reporter line (Ai9), showing hippocampal area CA1 immunolabelled for GFAP, tdTomato and NeuN; scale bars, 160 μm (top), 25 μm (bottom). ( F ) Quantification of % cells that are positive for tdTomato amongst NeuN-labelled cells in stratum pyramidale (left) and SR (middle) and GFAP-labelled cells (right).

Journal: bioRxiv

Article Title: Astrocyte GluN2C NMDA receptors control basal synaptic strengths of hippocampal CA1 pyramidal neurons in the stratum radiatum

doi: 10.1101/2021.05.28.446253

Figure Lengend Snippet: ( A ) Top: patch RT-PCR strategy. Bottom: representative voltage responses to current steps in CA1 pyramidal neuron (far left, black), and current responses to voltage steps in astrocytes in stratum radiatum (SR, green), stratum oriens (SO, yellow), and stratum lacunosum moleculare (SLM, blue). Scale bars: neuron, 40 mV, 500 ms; astrocytes, 6 nA, 200 ms. ( B ) Summary of I-V curves of astrocytes obtained before collecting RNA. N = 39 cells, 36 slices, 9 mice for each layer. ( C ) Summary of RT-PCR analysis for mRNA encoding indicated NMDAR subunits: GRIN1 (1), GRIN2A (2A), GRIN2B (2B), and GRIN2C (2C). Control samples (no patch) were obtained by inserting the electrode into the slice without patching cells. GRIN2D levels were undetectable in all cells examined (data not shown). ( D ) Western blots of co-immunoprecipitations performed using adult mouse hippocampal extracts with the indicated antibodies where anti-Cre antibody is used as a negative control. The blots are probed for GluN2A/2B (top row), GluN2C (middle row) or GluN1 (bottom row). ( E ) Representative brain sections of GRIN2C -Cre mice crossed to a tdTomato reporter line (Ai9), showing hippocampal area CA1 immunolabelled for GFAP, tdTomato and NeuN; scale bars, 160 μm (top), 25 μm (bottom). ( F ) Quantification of % cells that are positive for tdTomato amongst NeuN-labelled cells in stratum pyramidale (left) and SR (middle) and GFAP-labelled cells (right).

Article Snippet: Antibodies used were: mouse anti-NR1 (Synaptic Systems #114011), rabbit anti-GluN2A/2B (Synaptic Systems #244003), rabbit anti-GluN2C (generously provided by Dr. Masahiko Watanabe or purchased from Frontier Institute #GLURE3C-RB-AF270), mouse anti-Cre (Merck Millipore clone 2D8) and mouse anti-BirA (Novus biologicals #NBP2-59939).

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Negative Control